Journal: Scientific Reports

Article Title: Effect of olfactory stimulation from aromatherapy on the autonomic nervous activity during aerobic exercises

doi: 10.1038/s41598-024-61732-w

Figure Lengend Snippet: Mean values and their respective standard deviations of indices in the HRV analysis during rest, exercise, and recovery (Rec), obtained from the aroma and control conditions. These indices at rest were standardized to zero, and their changes were compared. *Values with significant differences than at rest (Friedman test followed by the Bonferroni correction; P < 0.05); †Values with significant differences than at rest (One way ANOVA for repeated measures followed by the Bonferroni correction; P < 0.05); #Values with significant difference than during aroma and control conditions (paired t-test; P = 0.028).

Article Snippet: HR and HRV indices were analyzed using HRV analysis software (Lab Chart 8, Nihon Bioresearch Inc., Japan) at the following time intervals: Rest (rest 5–10 min), Exercise (exercise 15–20 min), Rec1 (recovery period 0–5 min), Rec2 (recovery period 5–10 min), and Rec3 (recovery period 10–15 min).

Techniques: Control

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: CRISPR-Cas9 correction of a nonsense mutation in LCA5 rescues lebercilin expression and localization in human retinal organoids

doi: 10.1016/j.omtm.2023.05.012

Figure Lengend Snippet: Generation of an isogenic control by CRISPR-Cas9 correction using S.p. Cas9 Nuclease V3 of the homozygous variant LCA5 c.835C>T in patient-derived iPSCs (A) Schematic of isogenic pair generation and differentiation into retinal organoids. (B) Two guide RNAs and respective single-stranded oligodeoxynucleotide (ssODNs) (gRNA1 in green, gRNA2 in magenta) targeting the region surrounding the nonsense LCA5 c.835C>T variant in the patient iPSCs genome to restore the wild-type sequence. Protospacer adjacent motifs (PAM) for each gRNA are in gray. Nucleotide substitutions in ssODN templates are in color, with synonymous nucleotide substitutions signed with arrows. (C) Total (NHEJ+HDR) and LCA5 c.835C>T correction (HDR) editing efficiencies of gRNA1 and gRNA2 separately or supplemented with 30 μM IDT small-molecule HDR Enhancer (SD is standard deviation, n = 2 experiments, except for gRNA2+HDR Enhancer condition where n = 1). (D) Sanger sequencing of the patient and gene-corrected control clones #1 and #2 that were generated using ssODN1. The c.835C>T substitution is in red, and the three designed PAM-blocking silent nucleotide substitutions are in gray.

Article Snippet: Of the two designed gRNAs, in silico analysis showed that gRNA1 had specificity scores (78/100 for gRNA1 vs. 31/100 for gRNA2) and on-target cutting frequency (92/100 for gRNA1 vs. 31/100 for gRNA2), according to the Zhang lab MIT CRISPR design software ( https://www.benchling.com/ ).

Techniques: Control, CRISPR, Variant Assay, Derivative Assay, Sequencing, Standard Deviation, Clone Assay, Generated, Blocking Assay